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In vitro proliferation analysis of adult human CFs in response to 10−8 M CT-1 stimulation with or without 10−6 M BNP or CNP was determined by BrdU incorporation (A) and in response to 10−6 M CNP with and without the NPR-A/NPR-B antagonist, HS-142-1 and NPR-C antagonist, cANF(4–23) was determined by BrdU incorporation (B). Values are the mean of three samples per treatment from four experiments. Passages 1–4 were used. P<0.05.
