Figure 1.
ERK1/2 is activated in glial cells following LPC-induced demyelinating injury. A, B, Western blot analysis and quantification of microdissected tissue from the lesioned area of corpus callosum from WT mice at 2, 7, 14, and 28 dpl. Quantification was performed using ImageJ software (n = 3 mice per time point). C, Lesioned tissue from corpus callosum was immunostained for pERK1/2 at 2,7, and 14 dpl. Double labeling at 14 dpl for pERK1/2 with NeuN was used to mark neuronal nuclei (D), with GFAP to mark astrocytes (E), with IBA1 to mark macrophages and microglia (F), and with CC1 to mark mature oligodendrocytes (G). H, Higher power magnification shows colocalization of pERK1/2 in some, but not all, CC1+ oligodendrocytes at 14 dpl. Dashed lines highlight lesioned area. White arrow points to an oligodendrocyte-expressing pERK1/2 inside the lesion. Values are expressed as ±SEM, *p ≤ 0.05, **p ≤ 0.01. Scale bars: C–G,100 μm; H, 12.5 μm.