Table 1B. qPCR validation of highly enriched or depleted miRNAs in synaptoneurosomes from the whole brain.
Six candidates were selected for qPCR after MAANOVA. Note that relative enrichment resulted higher in the qPCR assays likely due to the sensitivity of the method. P values represent significant statistical differences calculated by paired Student's t test (n=3).
| miRNA | Microarray (SN/Total) | qPCR (SN/Total) | P-val (qPCR) |
|---|---|---|---|
| miR-434 | 1.36 | 2.5±0.17 | 5.00×10-4 |
| miR-132 | 1.28 | 2.29±0.48 | 8.30×10-2 |
| miR-181a | 1.17 | 2.14±0.17 | 5.40×10-2 |
| miR-126 | 0.33 | 0.06±0.01 | 2.24×10-7 |
| miR-145 | 0.33 | 0.06±0.06 | 3.25×10-7 |
| miR-143 | 0.08 | 0.05±0.01 | 8.86×10-6 |