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. Author manuscript; available in PMC: 2015 Nov 7.
Published in final edited form as: Nature. 2015 Mar 4;521(7550):85–89. doi: 10.1038/nature14179

Figure 1. The GP and bordering NB contain two cell types that project to FC.

Figure 1

a. Left, sagittal section from a Drd2-EGFP mouse injected with retrobeads into FC. Center, retrobead+ neurons (inset and circle-highlighted) in a medial section of GP. Right, retrobead+ overlay from 3 separate injections, spanning ~300 μm and excluding ventral basal forebrain. ac, anterior commissure; ic, internal capsule; Str, striatum; VP, ventral pallidum. b–d. Anterograde labeling of ChAT+ and ChAT− GP-FC axons. b. Coronal sections from a ChAT i-Cre mouse injected in GP (EDFig. 3g) with rAAVs DIO-EGFP (Cre-On) and FAS-tdTomato (Cre-Off) sampled from a whole-brain reconstruction. M1, primary motor cortex; Rt, thalamic reticular nucleus; STN, subthalamic nucleus; SNr, substantia nigra reticulata. c. Anterior-posterior distribution of normalized ChAT+ and ChAT− cortical axon densities (2 mice, solid and dotted lines). d. Left, GP-FC axons across layers in anterior M1. Right, normalized average fluorescence from dotted box. e. ChAT+ and ChAT− GP-FC cells are distinguishable in acute brain slices following green retrobead injection in FC of ChAT i-Cre;Rosa26lsl-tdTomato mice. f. Example membrane voltage (Vm) traces for GP-FC cells following positive (ChAT+:1.7; ChAT−:0.9 nA) and negative (ChAT+:−0.2; ChAT−: −0.1 nA) current injections (500 ms) to determine maximum firing rates and hyperpolarized membrane properties. Resting Vm is indicated. g. Whole-cell spontaneous firing rates (n=45 ChAT+ cells, n=35 ChAT−, 10 mice). Asterisk, P<0.05 (Mann-Whitney).