FIGURE 1.
A library of stable human VH single domains derived from the HEL4 model domain (Garvan I). A, amino acid sequence of HEL4 model domain, DP47 germ line, and Garvan I library with numbering according to Kabat, with CDR1, CDR2, and CDR3 regions highlighted in green, pink, and blue, respectively. B, schematic representation of human VH single domain with CDR3 region highlighted. C, colloidal stability (aggregation resistance) of HEL4-DP47 chimeras, as measured by retained binding to superantigen after heating to 80 °C (mean, n = 3). D, colloidal stability of Garvan I library, as measured by retained binding to superantigen after heating to 80 °C. E, selection of binders.