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. Author manuscript; available in PMC: 2015 May 5.
Published in final edited form as: Nature. 2014 Dec 10;517(7536):583–588. doi: 10.1038/nature14136

Extended Data Figure 5. The effect of guide and SAM-component dilution on target activation.

Extended Data Figure 5

a, The results for dilution of sgRNA 2.0 on target activation. b, The result for dilution of sgRNA 1.0 on target activation. # denotes an activation of < 2-fold at 1× guide dilution. c, Effect of MS2-p65-HSF1 and dCas9-VP64 dilution, at 1:1, 1:4, 1:10, and 1:50 of the original dosage for each component, on the effectiveness of transcription up-regulation. The amount of sgRNA expression plasmid was kept constant. d, Effect of diluting all three SAM components (dCas9-VP64, MS2-p65-HSF1, and sgRNA) at 1:4, 1:10, and 1:50 of the original dosage for each component. Fold up-regulation is calculated using GFP-transfected cells as the baseline. Error bars indicate S.E.M. and N = 3 for all figures.