Figure 5. Citron-K and Gag colocalize to citron-K-induced cytoplasmic compartments.
(A and C) 293T cells were transfected with GagGFP or GagGFP/Gag in a 10:1 ratio and protein localization was determined by confocal microscopy. GagGFP localizes primarily to the plasma membrane in the absence of exogenous citron-K. (B and D) 293T cells were co-transfected with GagGFP or GagGFP/Gag combination and citron-K. The localization of GagGFP is altered in the presence of citron-K. Instead of localizing to the plasma membrane, GagGFP localized intracellularly with citron-K into ‘citron-induced cytoplasmic compartments’ (indicated by arrows). N; nucleus. Bars represent 17.5 μm.
