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. Author manuscript; available in PMC: 2016 May 1.
Published in final edited form as: Cancer Res. 2015 Feb 25;75(9):1908ā€“1921. doi: 10.1158/0008-5472.CAN-14-2394

Figure 5. TGFBR3 KD is necessary and sufficient to recapitulate much of an intermediate EMT.

Figure 5

SUM149PT cells expressing constitutive miR-424 assayed for (A) TGFBR3, ST3GAL5 and TMPRSS4 by IB and (B) TGFBR3 mRNA by qPCR. (C) Luciferase reporter using 3ā€™ UTR of TGFBR3 co-transfected with 5 nM miR-424 mimics. Mutant ā€“ mutated miR-424 seed binding site in reporter construct. Two-way ANOVA with Bonferroni post-test. (D) QPCR, with two-tailed unpaired t-test, and (E) IB for EMT markers in SUM149PT cells with stable TGFBR3 KD. (F) IB for EMT markers and phospho-ERK1/2 in SUM149PT cells overexpressing miR-424 and a TGFBR3 rescue construct. Representative actin show for (F). SEM shown, nā‰„3; *p<0.05, **p<0.01, ***p<0.001.