Figure 4. PARP inhibitor-resistant cells are sensitive to NF-κB inhibition.
Line chart shows sensitivity to BAY 11–7082 in parental or PARP inhibitor-resistant UWB1.289 (A) or HCC1937 cells (B). Error bar shows standard deviation of three independent experiments. Line chart shows sensitivity to bortezomib in parental or PARP inhibitor-resistant UWB1.289 (C) or HCC1937 cells (D). Error bar shows standard deviation of three independent experiments. The parental and PARP inhibitor-resistant UWB1.289 or HCC1937 cells were treated with bortezomib (500pM) for 24 hours or TNFα (100ng/ml) for 20 minutes and stained with anti-p65 antibody. Untreated cells were also stained as control. Histogram shows percent of cells with p65 in nucleus in parental or PARP inhibitor-resistant UWB1.289 (E) or HCC1937 (F) cells. p65 nuclear translocation was presented as % of cells with intensity contrast: (nuclear intensity – cytoplasmic intensity)/(nuclear intensity + cytoplasmic intensity) ≥ 1. Error bar shows standard deviation of three independent experiments. Line chart shows the sensitivity to PARP inhibitor (olaparib) with bortezomib (30nM for UWB1.289 and 500pM for HCC1937) in parental or resistant UWB1.289 (G) or HCC1937 (H) Error bar shows standard deviation of three independent experiments.