Fig. 2.
Young AS mice have abnormal motor activity compared with wild-type littermates in open-field arena and hindlimb-clasp assay. (A) Total distanced traveled in an open-field arena at PND 21 is compared between wild-type and AS littermates on a B6 (n: WT = 11; AS = 4) and Hybrid (n: WT = 9, AS = 10) genetic background. (B) Hindlimb-clasp behavior is compared between wild-type and AS littermates at PND 30 on a B6 (n: WT = 7; AS = 5) and Hybrid (n: WT = 7; AS = 6) genetic background. Severity score for hindlimb clasp was determined by the average time spent clasping during two 20-s trials (see Materials and Methods). Statistics for both panels: Wild-type and AS littermates on B6 or Hybrid backgrounds were analyzed individually with an unpaired, two-tailed t test before plotting on a single graph. *P < 0.05, ***P < 0.0001. Error bars represent SEM.