Fig. 1.
Loss of the maternally inherited Ube3a gene leads to abnormal USV production. (A–D) The total number of USVs emitted from maternally isolated mouse pups throughout postnatal development. (A–C) Wild-type and AS littermates are compared on a 129 (n: WT = 12; AS = 15), B6 (n: WT = 15; AS = 18), and Hybrid (n: WT = 24; AS = 22) genetic background, respectively. (D) Mutation of the paternal copy of Ube3a (PAT mice) does not lead to changes in USV production (n: WT = 11; PAT = 13). Statistics for A–D: Two-way, nonrepeating-measures ANOVA; Bonferroni correction for multiple comparisons. *P < 0.05, **P < 0.01, ***P < 0.0001. Error bars represent SEM.