Figure 2. Quantification of intensity profiles along the lengths of stereocilia.
(a) Still frames from a live-imaged hair cell with stable localization of EGFP-β-actin in the distal tip compartment. Dashed lines (orange) indicate stereocilia that were line-traced every 8 h beginning 24 h post transfection with pEGFP-β-actin. Images without line traces are available in Supplementary Fig. 1. (b) Line traces from stereocilia 1 and 2 demonstrate increasing levels of EGFP-β-actin in the cuticular plate and distal tip compartment, but not along the stereocilia shaft and confirm steady-state tip localization. Over the course of the experiment, additional stereocilia move in close proximity of stereocilium 2 (arrows) (a), resulting in extra peaks in the line traces that correspond to the distal tip of the second stereocilium. Scale bar, 5 μm.