FIGURE 6.
Protection in Parkin KO mice may be due to increased hepatocyte proliferation. A, WT and Parkin KO mice were treated with 500 mg/kg APAP or saline control for 6 h, and total liver lysates were analyzed by Western blot. B, densitometry quantification of Western blots for cyclin D1. Data shown are means ± S.E. (n = 4 per group). Results were normalized to Gapdh. C, representative images from PCNA staining are shown (arrows represent PCNA-positive hepatocytes). D, PCNA staining quantification. Results are presented as % PCNA-positive hepatocytes. Data shown are means ± S.E. (n = 3, ×100 magnification, no significant differences between groups). Six images were quantified per liver tissue. E, mice were treated as in A, and total liver lysates were analyzed by Western blot. F, densitometry quantification of Western blots for PCNA. Data shown are means ± S.E. (n = 3–4 per group; *, p < 0.05). Results were normalized to β-actin.