FIGURE 3.
Mitophagy occurred in both WT and Parkin KO mice after APAP treatment. A, representative EM images are shown from WT and Parkin KO mice treated with APAP (500 mg/kg) with or without CQ (60 mg/kg) for 6 h. An enlarged image of mitophagosomes is shown from the dotted line boxed area (arrows, mitophagosomes; M, mitochondria; N, nucleus). B, quantification of EM images. Data shown are means ± S.E. (n ≥ 2 mice per group, at least 10 images quantified per group; *, p < 0.05, no significant differences between WT and Parkin KO mice). C, WT and Parkin KO mice were treated with 500 mg/kg APAP or saline control for 6 and 24 h, and total liver lysates were analyzed for mitochondrial protein degradation by Western blot. Gapdh was used as a loading control. Results for two individual mice are shown. D and E, densitometry quantification of Western blots for Tom20 and cyclophilin D. Data shown are means ± S.E. (n = 4 for each group, no significant differences among groups). Results were normalized to Gapdh.