Figure 2.
Nuclear receptor agonist treatment increases MerTK and Axl receptor expression in microglia. At 24 h after addition of bexarotene, N9 microglia were incubated in the presence or absence of 10 ng/ml LPS for an additional 18 h before analysis of MerTK mRNA levels (A) and Axl mRNA levels (B). Protein levels of MerTK in primary microglia were evaluated by Western analysis 18 h after treatment with 50 nm bexarotene or 500 nm GW3965 (C, quantified in D). Protein levels of MerTK in N9 microglia were probed via Western blot 18 h after treatment with 50 nm bexarotene, 100 nm pioglitazone, 100 nm GW3965, or 100 or 500 nm GW0742 (E, quantified in F). Data represent three separate experiments. *p < 0.05. **p < 0.01. ***p < 0.001.