Figure 3.
Glutamatergic and dopaminergic neurons of the rat VTA. A. TH-immunoreactivity (brown) showing the SNc (left) and VTA (middle). B-C. Higher power images of VTA. TH-immunoreactivity (B,C) and VGluT2 mRNA (B’,C’) are displayed as brown reaction product (TH-immunoreactivity under bright field) and aggregates of silver grains (white grains under dark field or black grains under darkfield). D. High power image of selected PBP(lat) region in C. E. High power image of selected PBP(med) region in C. F. High power image of selected RLi region in C. High power image of selected IF region in C. Green arrows indicate VGluT2-only neuron. Yellow arrows indicate VGluT2-TH neuron. Blue arrowheads indicate TH-only neuron. fr - fasciculus retroflexus. Figure modified from Yamaguchi et al., 2011.