Skip to main content
. Author manuscript; available in PMC: 2015 Apr 9.
Published in final edited form as: Biochem J. 2010 Dec 15;432(3):525–534. doi: 10.1042/BJ20100862

Figure 5. Deletion of one of the intramolecular interaction sites in ALIX increases ALIX association with the membrane.<.

Figure 5

br>(A–C) HEK293 cells were transfected with an expression vector for WT (A), ΔP2 (B) or ΔTDS (C) GFP-ALIX. Crude cell lysates were fractionated by membrane flotation centrifugation, and proteins in collected fractions were immunoblotted with antibodies for each of the indicated proteins. M indicates membrane protein fractions; S indicates soluble protein fractions.

(D) ALIX in M and S fractions were quantified, and the percentages of total ALIX in each fraction were calculated. Error bars represent standard deviations from three separate experiments.