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. 2015 Apr 2;467(Pt 2):345–352. doi: 10.1042/BJ20141502

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© 2015 This is an Open Access article distributed under the terms of the Creative Commons Attribution Licence (CC-BY)(http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution and reproduction in any medium, provided the original work is properly cited.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution and reproduction in any medium, provided the original work is properly cited.

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(A) The crystal structure of Lys³³-linked triUb. (B) Representative diagram of how a Ub moiety in an ASU is arranged into triUb (left) and how Ile⁴⁴ and Ile³⁶ patches are located in Lys³³-linked triUb (right) (C) Superposition of the distal Ub moieties (white) of Lys³³-linked diUb and Lys³³-linked triUb, shown in cartoon and surface representations. Hydrophobic patches are coloured as in Figure 3D. Only two Ub moieties are shown for Lys³³-linked triUb. In comparison with the proximal Ub of Lys³³ diUb (teal), the proximal Ub of Lys³³ triUb (orange) moves approximately 65° from to the more open conformation. (D–F) A semi-transparent surface of Lys³³ triUb (D), Met¹ diUb (E) and Lys⁶³ diUb (F) coloured as in Figure 3D (PDB 2W9N, 2JF5 [13]).