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. 2015 Mar 30;6:6623. doi: 10.1038/ncomms7623

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(a) CD45⁺ cells increased in the tumour area of EE-housed mice (n=4; *P<0.05 Student’s t-test). Representative immunofluorescence is shown on the right, at low (× 4, top, scale bar 1 mm) and high (× 20, bottom, scale bar 0.1 mm also for b and c) magnification. (b) CD69⁺ cells increased in the tumour area of EE-housed mice (n=4; **P<0.01 Student’s t-test). Representative immunofluorescence is shown on the right. (c) Analysis of NK1.1 cell infiltration in tumour, expressed as % NK1.1⁺ cells in the tumour area (±s.e.m.; *P<0.05 Student’s t-test; n=3–4 mice per condition). Representative immunofluorescence of NK1.1⁺ cells (green) 17 days after glioma-cell transplantation in mice housed in SE or EE are shown on the right. (d) CD3⁻/NK1.1⁺cells increased in the brain hemisphere injected with glioma on housing in EE (n=6; *P<0.05 Student’s t-test). Representative FACS analysis of CD3⁻/NK1.1⁺ cells is shown on the right. (e) Percentage of CD69⁺ cells in the CD3⁻/NK1.1⁺ cell population obtained from the brain of SE or EE mice (n=6; *P<0.05, Student’s t-test). Representative FACS analyses are shown below. (f) Mean fluorescence intensity (MFI) of CD69 staining in CD3⁻/NK1.1⁺ cell population obtained from the brain of SE or EE mice (n=6; *P<0.05, Student’s t-test). (g) Analysis of tumour volume (expressed as mm³±s.e.m.) in vehicle and NK1.1 Ab-treated mice, housed in SE or EE; n=5; *P<0.05, two-way ANOVA). (h) Kaplan–Meier curve of glioma-transplanted mice exposed to SE or EE on NK1.1 Ab treatment; n=5; log-rank test **P<0.01.