Fig. 7.
Comparative Western blot analysis of AMPK, P-AMPK, and PGC-1α in H9c2 and HL-1 cells. A–C: Whereas a similar level of AMPK was found in both cell lines, H9c2 cells demonstrate significantly higher amount of phospho-AMPK (activated, P-AMPK). In contrast, higher level of PGC-1α was found in HL-1 cells as compared with H9c2 cells. A: Short hypoxia (2 h) resulted in significant activation of AMPK to P-AMPK, both in H9c2 and in HL-1 cells. B: Treatment with 1 mM of activator of AMPK metformin had no effect. C: Treatment with higher (4 mM) concentration of metformin showed an activation of AMPK, more visible after treatment with specific activator A769662 (0.1 mM, 8 h).