Figure 3.
NLRP3 knockdown improved neurological functions and reduced brain edema at 24 and 72 hours following intracerebral hemorrhage (ICH). (A) Modified Garcia test at 24 and 72 hours following operation in sham, ICH, scrambled small interfering RNA (siRNA; ICH+siCtrl), and NLRP3 siRNA mixture (ICH+siNlrp3s) groups at 12 hours following operation. (B, C) Brain edema at 24 hours (B) and 72 hours (C) following operation in sham, ICH, scrambled siRNA (ICH+siCtrl), and NLRP3 siRNA mixture (ICH+siNlrp3s) groups at 12 hours following operation. Brain sections (4mm) were divided into 4 parts: ipsilateral basal ganglia (Ipsi-BG), ipsilateral cortex (Ipsi-CX), contralateral basal ganglia (Cont-BG), and contralateral cortex (Cont-CX). Cerebellum (Cerebel) is the internal control; n=6–13 mice per group. Error bars represent median±25th to 75th percen-tiles (A) or mean±standard error of the mean (B, C). #p<0.05 vs sham, *p<0.05 vs ICH, @p<0.01 vs ICH+siCtrl.