Fig. 3.
Localization of Ang-2 and Pax7 proteins in cardiotoxin-injured and regenerating TA muscle. A–E: immunostaining for Ang-2 protein in noninjured (A, B) and injured TA muscles on day 1 (C, D), and day 14 (E) postinjury. Positive staining is indicated by bright red color (black arrows). False positive staining is indicated by pink staining. F: immunofluorescence staining for Ang-2 protein in blood vessels of noninjured TA muscle (white arrows). Black scale bars, 50 μm. G–I: immunofluorescence staining for Pax7 (red, G), Ang-2 (green, H), and DAPI (blue, I) in recovering (14 days postinjury) TA muscle section. White arrows point to Ang-2-positive Pax7-positive cell. White scale bars, 25 μm.