Fig. 2.
Localization of Ang-1 and Pax7 proteins in cardiotoxin-injured and regenerating TA muscle. A–F: immunostaining for Ang-1 protein in noninjured (A, B) and injured TA muscles on day 1 (C), day 3 (D), day 7 (E), and day 14 (F) postinjury. Positive staining is indicated by bright red color (black arrows). False positive staining is indicated by pink staining (blue arrows). Black scale bars, 50 μm. G and H: merged images of immunofluorescence staining for laminin (red) and Ang-1 (green) in noninjured TA muscle. White arrows point to Ang-1-positive cells. White scale bars, 25 μm. I: merged image of immunofluorescence staining for Pax7 (red) and Ang-1 (green) proteins in noninjured TA muscle. White arrows point to Ang-1-positive Pax7-positive cells. White scale bar, 25 μm. J–L: immunofluorescence staining for Pax7 (red, J), Ang-1 (green, K) and DAPI (blue, L) in recovering (14 days postinjury) TA muscle section. White arrows point to Ang-1-positive Pax7-positive cell. White scale bars, 25 μm.