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. 2015 Mar 24;6:6555. doi: 10.1038/ncomms7555

Figure 6. Crystal-mediated XOR activation induces the PI3K–AKT–mTOR pathway and mitochondrial ROS production.

Figure 6

(a) Colocalization in BMDM of fluorescein isothiocyanate-labelled anti-XOR antibody, Mitotracker and counterstained with 4,6-diamidino-2-phenylindole (DAPI). Images visualized at × 630. Scale bars are indicated. Results are representative of several fields of view with similar results after 6 h of stimulation. (b) Autophagic vesicle formation in unstimulated BMDM after 2 h treatment were detected by LC3B Antibody labelled with TRITC, and counterstained with DAPI. Images were visualized at × 400 and are representative of several fields of view with similar results. (c) Corrected total cell fluorescence (CTCF) was calculated for each cell in multiple fields of view for LC2B/TRITC red fluorescence. Results are expressed as mean±s.e.m. and significance was determined at *P≤0.05 and ****P≤0.0001 by ANOVA. NP, not performed. NS, not significant.