Figure 3. Crystal-induced IL1β secretion is mediated by ROS.
In vitro BMDM (Pam3Cys primed) were pretreated with XOR inhibitors: febuxostat, 200 μM and allopurinol, 500 μg ml−1 and stimulated with OCP or MSU at 250 μg ml−1, Alum-500 μg ml−1 and HZ-200 μg ml−1. (a) Superoxide was measured with dihydroethidium (DHE), results expressed as fold increase in relative fluorescence units (RFUs) at T=120 min over T=0. (b) ROS measured by luminol, data obtained by relative luminescence units (RLUs) at T=60 min. Data are representative of at least two experiments. Results are expressed as mean±s.e.m. Significance was determined at *P≤0.05, **P≤0.01, ***P≤0.005 and ****P≤0.0001 by the t-test for all panels.