Figure 1.
Characterization of MSCs derived from bone marrow and MSCs transduced with GATA-4 (MSCGATA-4). A, Second passage of basal bone marrow stem cells. B and C, Immunostaining of GATA-4 in MSCGATA-4 (B) and MSCNull (C). GFP was expressed in both MSCNull and MSCGATA-4, but GATA-4 was only expressed in MSCGATA-4. D, Real-time PCR results show that the expression of miR-19a and miR-451 was up-regulated in MSCGATA-4 compared with MSCNull. E, Expression of miR-19 was down-regulated in MSCs that were transfected with GATA-4-siRNA (MSCsi-GATA-4) compared with MSCs transfected with the negative control siRNA (MSCsi-NC). *, p<0.05 vs MSCNull and #, p<0.05 vs MSCsi-NC.
