Fig. 3.
A population of mreBA325P cells contains a broad distribution of widths and Venus-MreBA325P localizes predominantly in thin regions.
Histograms of cell widths for large populations of wild-type and mreBA325P cells expressing Venus-MreB and Venus-MreBA325P respectively. Cells were divided into 1-pixel (~65 nm)-thick segments along their long axes, and segments were binned according to width. Note that the average number of segments per cell was greater for mreBA325P because these cells were often longer than wild-type. The mean Venus-MreB intensity of segments in each width bin was calculated and is overlaid on top of the histograms. Error bars represent the standard error of the mean.