Figure 5. Rapamycin augments systemic myxoma virus treatment of B16F10-LacZ lung metastases.
(a) Schematic representation of treatment schedule; (b) The stained lungs were photographed, the lobes were separated, and each lung was separately observed under a dissecting microscope and (c) the number of stained surface metastases counted. The results represent those from 9–14 animals per group. (d) Measurement of the mean size of tumors. Fixed lungs were placed between two slides, and the diameters of individual tumors were measured using OpenLab software. The measurements from each lung were compiled and analyzed. The results are from six animals per treatment. (e) Estimation of tumor burden. One lobe from each animal lung was removed prior to fixation and homogenized. The extent of β-galactosidase activity was determined using a colorimetric assay. The results are representative of six animals per group. The P values result from an unpaired t-test. Ffu, focus forming units. IP, intraperitoneal; IV, intravenous.