Figure 3.
Time course of TGF-β1, TGF-β2, and TGF-β1R gene expression in IgE-stimulated LAD2 cells. LAD2 cells were stimulated with IgE receptor cross-linking for 0, 0.5, 2, 4, 6, and 24 hours, and the gene expression of TGF-β1, TGF-β2, and TGF-β1R was measured by real-time RT-PCR. Data represent three independent experiments. *P < 0.005, **P < 0.01, and ***P < 0.05 compared with 0–h IgE by Student’s t test. GCN, gene copy number; normalized GCN, relative GCNs normalized to the two most stable housekeeping genes, GAPDH and EEF1A1.