Fig. 8.
Colocalization of PAI-1 and LRP-1 in U87MG glioma cells. U87MG cells were treated with either vehicle control, UCD74A, or UCD38B at 250 μM for 60 minutes. PAI-1 seen in green was immunostained with anti–PAI-1 primary and Alexa 488 secondary antibody. LRP-1 in red is detected by anti–LRP-1 primary and Alexa 594 secondary antibody (A). DAPI, 4′,6-diamidino-2-phenylindole . LRP-1 in green was associated with early endosomes immunostained with EEA-1 antibody (red) (B). Colocalization of LRP-1 with late endosomes was detected with LAMP-1 antibody (red) (C). Scale bar is 50 μm.