Fig. 2.
Localization of PAI-1 in U87MG glioma cell. Intracellular localization of PAI-1 was visualized by confocal microscopy with an anti–PAI-1 antibody in U87MG cells that had been treated for 120 minutes with 250 μM of UCD38B, UCD74A, or vehicle (0.1% v/v DMSO). (A) Alexa 488 was used as a secondary antibody. MitoTracker and 4′,6-diamidino-2-phenylindole (DAPI) were used to visualize the mitochondria and nucleus, respectively, and the scale bar is 50 μm. Cytosolic (C) and mitochondrial (M) enriched fractions were separated by SDS-PAGE. PAI-1 was visualized on an immunoblot with Actin and voltage-dependent anion channel as cytosolic and mitochondrial marker proteins, respectively (B). Densitometry of PAI-1 expression, normalized to marker proteins. (C) is presented as the mean ± S.D. of n = 3 with ***P < 0.001.