Figure 3.
Schematic diagram of the CLSM. The excitation laser beam light path (A) and emission light path (B). The solid blue lines in (A) represent the excitation laser that is focused onto the specimen. The solid green lines in (B) show that emission light from the focal plane passes through the pinhole aperture and is detected by the PMT. However, the dashed gray lines show that out-of-focus light will be blocked, will not pass through the pinhole, and will not be detected by the PMT. Reprinted with permission from Methods in Cell Biology V123, p113-134, 2014.