Figure 3.
TH2 cell–mediated inflammation in the lungs of mice depleted for Id2 and Id3 expression in Treg cells. (a) May-Giemsa staining images of cells (left) and cell numbers (right) in BAL fluid derived from 6–8-week-old control (Ctrl) or Id2fl/flId3fl/flFoxp3Cre mice (left). Green, red and blue arrowheads indicate macrophages (MF), lymphocytes (Lym) and eosinophils (Eos), respectively; Neu, neutrophil. Insets show magnification of boxed areas. Original magnification, ×40 (×120 in insets). (b) Numbers of hematopoietic cells (CD45+), macrophages (MF), eosinophils (Eos), basophils (Bas), neutrophils (Neu), mast cells (Mas), T cells (T) and B cells (B) isolated from the left lobes of the lungs derived from Id2fl/flId3fl/flFoxp3Cre mice (left). Numbers of type 2 innate lymphoid cells (ILC2; CD45+LineageCD25+IL7R+Sca1hiThy1.2hi) isolated from the left lobes of the lungs derived from Id2fl/flId3fl/flFoxp3Cre mice (right). (c) Flow cytometric analysis of CD19 versus TCRβ expression of cells derived from the LNs isolated from 6–8-week-old control (Id2fl/flId3fl/flFoxp3Cre/+) or Id2fl/flId3fl/flFoxp3Cre/Cre mice. Adjacent panels indicate CD11c versus SiglecF, Fcer1 versus SiglecF and Mac-1 versus Gr-1 expression, gated on the TCRβ−CD19− compartment derived from 6–8-week-old control (Id2fl/flId3fl/flFoxp3Cre/+) or Id2fl/flId3fl/flFoxp3Cre/Cre lymphocytes isolated from subcutaneous LNs. Numbers in plots indicate percent CD19−TCRβ−, CD11c−SiglecF+, Fcer1+SiglecF− and Mac-1+Gr-1+ cells. Right, percentages of eosinophils (SiglecF+CD11c−), basophils (Baso; Fcer1hiSiglecF−) and neutrophils (Neut; Mac-1hiGr-1hi) in subcutaneous LN cells and splenocytes. Data are representative of four experiments (a,c; mean ± s.d.; in a, n = 8 independent biological replicates; in b, control n = 5, Id2fl/flId3fl/flFoxp3Cren = 4 independent biological replicates; in c, control n = 6, Id2fl/flId3fl/flFoxp3Cren = 9 independent biological replicates). *P < 0.05, **P < 0.01 (two-tailed unpaired Student’s t test).