FIGURE 7.
Reversed expression pattern of FXR and CCNA2 in cell lines and mice. A and B, expression of CCNA2 was quantified in Huh7 (A) and HCT116 (B) cells after 24 h CDCA treatment (50, 100, and 150 μm). C, protein level of CCNA2 was determined by Western blot after 48 h treatment of 100 μm CDCA. D–F, the mRNA level of miR-22 and protein level of CCNA2 were determined in the livers and ileums of 3-month-old FXR KO mice. G, Ki-67 staining of liver and colon sections obtained from WT and FXR KO mice (magnification, 40×). The data are presented as the means ± S.D. *, p < 0.05; **, p < 0.01; ***, p < 0.001.