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. 2014 Sep 9;39(3):424–429. doi: 10.1038/ijo.2014.153

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Intra-colonic administration of propionate increases portal vein plasma PYY and GLP-1 levels in vivo in mice via an FFA2-dependent mechanism. Blood samples were collected from the portal vein 5 min after an intra-colonic injection (300 μl) of propionate (180 mmol l⁻¹) or saline (matched for pH and sodium content) in isoflurane-anaesthetised FFA2 knockout (−/−) mice or WT littermates (+/+). Data represent means±s.e.m. (n=5–7 per group). Significance was determined using one-way ANOVA (a, F=2.457, P=0.094; b, F=2.660, P=0.076) with a Bonferroni post hoc test (*P<0.05).