Figure 1. Cyclopentenone prostaglandins protect cells from LT-induced pyroptosis.
RAW264.7 cells (A) or Balb/cJ BMDMs (B) were treated with varying concentrations of prostaglandins for 30 min in a dose range where the compounds were non-toxic. Cells were then challenged with 1 μg/mL LT and viability was measured by MTT staining at 1.5–2 h post-LT. All values are average from two wells in one representative experiment. Each curve is representative of at least two and up to ten similar experiments, depending on the particular compound shown. (C) RAW264.7 cells were incubated with 15d-PGJ2 (50 μM, 30 min) followed by LT (1 μg/mL) for indicated times. Western blotting of cell lysates was performed with antibodies against the N-terminus of MEK1 and MEK3. The * indicates a cross-reactive protein band which acts as an internal equal loading control. Results represent three similar Westerns.