Figure 6. LncLSTR interacts with TDP-43 to regulate Cyp8b1 expression.
(A) Cyp8b1 promoter-driven luciferase activities in cells transfected with HNF4α expression vector in combination with vectors expressing either TDP-43 or lncLSTR. Negative control for HNF4α and TDP-43 is a pcDNA 6.2 vector expressing a yellow fluorescent protein, and negative control for lncLSTR is the empty pcDNA 6.2 vector.
(B) Luciferase activities driven by Cyp8b1 promoter (200bp) in cells transfected with HNF4α expression vector in combination with vectors expressing either TDP-43 or YFP control.
(C) In vitro binding of biotinylated Cyp8b1 promoter (-200-1) with recombinant TDP-43. A biotinylated Cyp8b1 promoter fragment (-1503--1303) was used as a negative control.
(D) In vitro binding of biotinylated Cyp8b1 promoter fragments as indicated with recombinant TDP-43.
(E) Chromatin immunoprecipitation of liver tissues of control and lncLSTR KD mice using an anti-TDP-43 antibody. Bands were amplified with specific primers for Cyp8b1 (targeting -392 to 1) or β-actin promoters.
(F) A working model where lncLSTR regulates a TDP-43/FXR/apoC2 pathway to control plasma TG clearance in mice.
Error bars represent SEM, *p<0.05.