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. Author manuscript; available in PMC: 2015 Aug 1.
Published in final edited form as: Nat Protoc. 2015 Jan 22;10(2):316–333. doi: 10.1038/nprot.2015.020

Figure 6. Doublet removal with the 6-choose-3 MCB scheme and single cell de-barcoding.

Figure 6

(a) Biaxial plot of event length × Ir-intercalator of events that were assigned a barcode, and of events that were left unassigned. (b) Percent of cells assigned by gating (green squares) or debarcoding (purple circles) versus percent of assigned cells that are CD4+CD8+ doublets. The different yields were acquired by variable event length × Ir-intercalator gates (green squares) or debarcoding threshold stringency (purple circles). The arrow indicates the debarcoding parameters used in Figures 5 and 6a.