Figure 2.
S100A4 and S100A11 are recruited to the site of injury before actin build-up. HeLa cells were transiently transfected to express (A) S100A4-GFP and S100A11-RFP or (B) S100A4-GFP and the F-actin reporter protein Utrophin-mCherry. The cells were injured focally by a pulsed laser (white arrow) and the response of the proteins was monitored live as the cells underwent repair in the same way as we described previously.13 The plot shows the kinetics of accumulation of individual proteins at the repair site (blue arrow) marked by the white box.