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. 2015 Feb 19;6:6249. doi: 10.1038/ncomms7249

Figure 2. TEM images of GUVs incubated with AP180/clathrin under different osmotic conditions.

Figure 2

(a) Hypotonic conditions. Scale bars, 1 μm (top row) and 200 nm (bottom row). (b) Isotonic conditions. Scale bar, 200 nm. (c) Hypertonic conditions. Scale bars, 1 μm (top row) and 200 nm (bottom row). (d) Statistics of GUVs appearance observed by TEM under different conditions. (e) Micrographs of clathrin lattices in isotonic conditions (scale bar, 200 nm) with a high-magnification image (scale bar, 100 nm (bottom)) and (f) micrographs of clathrin lattices in hypertonic conditions showing tabulation of the membrane (scale bar, 200 nm) with a high-magnification image (inset, scale bar, 100 nm). For electron microscopy experiments, n=372 (hypotonic), n=54 (isotonic) and n=134 (hypertonic) from at least three independent experiments.