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. Author manuscript; available in PMC: 2016 Jan 15.
Published in final edited form as: Cell. 2015 Jan 15;160(0):241–252. doi: 10.1016/j.cell.2014.12.032

Figure 3. Endothelial cells in the perivascular niche remodel to surround a single HSPC.

Figure 3

(A) 4 frames from time-lapse Movie S2 between 40-42 hpf (hours post fertilization:minutes). Upper row is merge of Runx:GFP+ HSPC (green, middle row) and kdrl:RFP ECs (red, lower row). A group of surrounding ECs (broken circle) remodel around a single HSPC soon after its arrival. (B) Higher magnification (60x) live image of single Runx:GFP+ HSPC surrounded by kdrl:mCherry ECs at 78 hpf (orthogonal views). (C) 3D rendered projection of scanning confocal image from fixed 80 hpf embryo. (i) merge, (ii) kdrl:mCherry ECs, (iii) Runx:GFP+ HSPCs, (iv) DRAQ5 nuclei, (v) kdrl:mCherry projection with 3D modeled green HSPCs and 5 blue surrounding EC nuclei (arrowhead indicates HSPC in EC surround). All views: dorsal up, ventral down. Scale bars: 10 μm. See also Figure S3.