Figure 7. Th1 and Th17 cells provide compartmentalized protection to C. albicans.
WT, Il6−/− and Batf3−/− mice were epicutaneously infected with Eno1-Ag. (A) The expression of IL-17A and IFN-γ by PMA and Ionomycin stimulated CD4+ TEα cells isolated from skin draining lymph nodes 4 days after infection is shown. (B) The indicated strains of mice were mock infected (white) or skin infected with SC5314. Mice were re-challenged in the skin with SC5314 9 days after spontaneous clearance of the initial infection. Fungal skin burdens of individual mice is shown as CFU/cm2. (C) CD4+ T cells were purified from skin draining LN and spleen of naïve WT, SC5314 infected Il6−/− or SC5314 infected Batf3−/− mice 7 days after infection and adoptively transferred into into naïve WT recipients. Recipients were skin infected with SC5314 1 day after transfer and CFU was assessed at the infected area 2 days later. (D) As in (B) except that mice were challenged by i.v. infection with 105 SC5314. (E) As in (C) except that mice were challenged by i.v. infection with 105 SC5314. Fungal burdens in the kidneys expressed as CFU/g is shown. Scales represents mean ± SEM. *P<0.05, **P<0.01, ***P<0.001.