FIG. 6.
The slow to fast MyHCIIA transition observed in Sol fibers of mice treated with FK506 does not lead to Six1/Eya1 nuclear enrichment. (A) The percentages of fibers expressing different MyHC isoforms in Sol and plantaris muscles of mice treated with FK506, as well as in those of untreated controls, were determined by immunohistochemistry assays performed on hindlimb muscle cross sections at the Gas-Sol-plantaris level (n = 2 mice per group). (B) Immunohistochemical analysis of Six1 and Eya1 expression on cross-sections of FK506-treated or control Gas and Sol muscles, revealing the absence of Six1 or Eya1 nuclear enrichment in Sol fibers of FK506-treated animals.