FIG. 3.
Six1 is enriched in fast-twitch muscle nuclei. (A to C) Immunohistochemistry assays of adult mouse muscle sections at the Sol-Gas level performed using polyclonal antibodies directed against Six1 (A), Six4 (B), and Six5 (C). (D) Northern blot experiment performed with 20 μg of total RNA, showing the accumulation of Six1 mRNAs (upper panel) in adult muscles together with the relative levels of 18S rRNA content (lower panel) present in the different samples analyzed. Lane 1, Gas; lane 2, extensor digitorum longus; lane 3, Sol. (E) Western blot experiment performed with 100 μg of total protein, showing the presence of Six1 (upper) and α-tubulin (below) in Gas (lane 1) and Sol (lane 2) muscles.