FIG. 2.
Mapping of eIF4G-binding site within 100K protein. 293T cells were transfected with plasmids as indicated, and then at 36 h posttransfection equal amounts of total protein lysates were incubated with eIF4G antiserum (eIF4G IP) or preimmune serum (Preim. IP). Immune complexes were resolved by SDS-10% PAGE, and associated proteins were detected by immunoblot analysis of endogenous eIF4G (anti-eIF4G) or Flag (anti-Flag). Typical results from at least three independent experiments are shown. IgG, immunoglobulin G; CCR, coiled-coil region.