FIG 8.
NS-EM analyses of B41 SOSIP.664 trimers in complex with Fabs. (A) The left side shows top and side views of a 3D reconstruction (gray) of VRC01-liganded B41 trimers with the high-resolution model of PGV04-liganded BG505 trimers docked (PDB code 3J5M). The gp120 subunits are in blue, V1V2 is in magenta, V3 in green, gp41ECTO in brown, and PGV04 in red. The right side shows the VRC01-liganded B41 trimer density (gray) superimposed on the volume map of the PGV04-liganded BG505 SOSIP.664 trimer (pink) (EMD-2427). (B) EM 2D class averages of VRC01-bound and b12-bound B41 SOSIP.664 trimers (left and right, respectively). (C) Projection matching (left) and Fourier shell correlation (right) for the VRC01-liganded B41 trimers. All images are of non-V3-clipped trimers produced in 293T cells and purified by the PGT145/SEC method.