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. Author manuscript; available in PMC: 2015 Feb 22.
Published in final edited form as: Cell Stem Cell. 2010 Apr 2;6(4):336–347. doi: 10.1016/j.stem.2010.03.001

Figure 2.

Figure 2

Floor Plate induction has an early, short temporal patterning window

(A) Schematic showing different time points of Sonic C25II additions during neural induction protocol.

(B-C) Heading on the left delineates the day Sonic C25II was added, heading on the top delineates when the assay was stopped. The earlier Sonic C25II is added, and the longer the cells are exposed to it, leads to very high percentages of FOXA2. (C) This result is quantified, *p<0.01 N=3. Scale bars, 200um, high mag, 50um.

(D) Extended treatment with Sonic C25II (9 days of exposure) does not yield increased FOXA2 induction.

(E) Schematic of optimal protocol for FOXA2 induction to be used for the rest of the study.