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Long term (30 day) EcoHIV infection combined with morphine increases cytokine expression in brain over infection alone. RNA was isolated from brain and analyzed via qPCR for IL-6 (A), IFN-γ (B), and IL-1β (C). N: Placebo=3, Morphine=5, EcoHIV=3, EcoHIV+morphine=5. *p<.05