Fig. 5.
Macrophage phagocytosis is decreased by EcoHIV infection and worsened in combination with morphine. Peritoneal macrophages were harvested from EcoHIV infected mice and cultured with FITC-dextran and the phagocytic index was calculated from fluorescence as a percentage of control (A). Peritoneal macrophages were incubated with FITC-dextran (green) and stained with phalloidin (Red) and DAPI (blue); shown are representative images (B) and quantification of multiple fields (C). RAW 264.7 macrophage cell line was incubated with FITC-dextran as in part A (D). This approach was repeated with S. aureus (E) or E. coli (F) opsonized particles to examine any bias between TLR2- or TLR4- mediated phagocytosis. N: all groups=6. *p<.05, **p<.01, ***p<.001