Fig. 4.
EcoHIV with morphine does not greatly alter proportions of immune cells within the gut. Gut cells were collected from homogenized distal SI (A,D,G), Cecum (B,E,H), or colon (C,F,I) and stained for FACS analysis. CD3+ T-cells were gated and values of CD4, CD8, or CD4/CD8 double positive were examined (A,B,C), with the only significant change in DP cells being higher in morphine, EcoHIV, and EcoHIV+morphine. CD19+ B-cells were examined as a percentage of live cells (D,E,F) and no significant change was observed. CD45+ monocyte cells were gated and values of CD11B, CD11C, or CD11B/CD11C double positive were examined (G,H,I), with the only significant change in large intestine DP cells in morphine, EcoHIV, and EcoHIV+morphine. Cells were gated for CD45+ and MHCII high to see CCR5 expression in macrophages, which saw trending increases at all locations, most notably in EcoHIV+morphine. N: Placebo, 4; morphine, 6; EcoHIV, 5; EcoHIV+Ms, 8. *p<.05